Dynamic Light Scattering
Nanoparticle and macromolecular sizing on the Wyatt Technology DynaPro Plate Reader II
Moore Analytical now offers dynamic light scattering (DLS) on the Wyatt Technology DynaPro Plate Reader II — a high-throughput, automated platform for measuring particle and molecular size, size distribution, and aggregation behavior directly in solution.
DLS measures the Brownian motion of particles suspended in a liquid and relates it, through the Stokes–Einstein relationship, to hydrodynamic size. It is a fast, non-destructive technique requiring no staining, drying, or sectioning, making it well suited to characterizing materials in their native, hydrated state.
What is measure
- Hydrodynamic radius from 0.5 nm to 1,000 nm (≈ 1 nm to 2 µm diameter)
- Particle size distribution and polydispersity index (PDI)
- Aggregation onset and the presence of higher-order aggregates
- Thermal stability via temperature ramps (4–85 °C): aggregation onset temperature (T<sub>onset</sub>) and unfolding/melting temperature (T<sub>m</sub>)
- Colloidal stability and protein–protein interaction parameter (k<sub>D</sub>)
- Batch-to-batch and formulation comparisons
Typical applications
- Sizing of nanoparticles, colloids, micelles, emulsions, vesicles, liposomes, and viral / non-viral delivery vectors
- Protein and biologic characterization: aggregation, stability, and formulation screening
- Detection and sizing of sub-micron contaminants, aggregates, or unwanted particulates in fluids and formulations
- Colloidal and shelf-life stability studies
- Incoming-material verification and quality control
- Investigation of turbidity, haze, or particle-related product failures
Sample requirements
Please contact us to confirm solvent compatibility, concentration range, and any filtration requirements before shipping. Minimum volumes are low, but supplying sufficient sample allows replicate measurements and improves confidence in the result.